Cisplatin decreases cyclin D2 expression via upregulating MIR-93 to inhibit lung adenocarcinoma cell growth

Abstract

MicroRNA s (miRNA s/miRs) serve important roles in the chemotherapeutic effect of anticancer drugs. To investigate the roles of miRNA s in cisplatin-induced suppression of lung adenocarcinoma cell proliferation, A549 cells were treated with different concentrations of cisplatin. An MTT assay demonstrated that cisplatin inhibited A549 cell proliferation in a dose-dependent manner. Cisplatin induced cell apoptosis and inhibited cell migration by increasing the levels of miR- 93, miR- 26a and miR- 26b. Furthermore, as an upstream factor, miR- 93 was proposed to regulate cyclin D2 expression in miR- 93-transfected A549 cells. Cisplatin also induced Bcl-2-associated X protein expression, and decreased that of Bcl-2 and c-Myc in lung adenocarcinoma cells. In vivo analysis further supported that cisplatin inhibited lung adenocarcinoma cell growth by regulating cyclin D2 and miR- 93 expression. In conclusion, our findings demonstrated that cisplatin could effectively inhibit lung adenocarcinoma cell proliferation by decreasing cyclin D2 expression via miR- 93.