Taxonomy, cytology and palynology of kaempferia pseudoparviflora (Zingiberaceae), a new and rare specie from Northern Thailand

Abstract

Background and Objective: Kaempferia pseudoparviflora is a new species from Thailand. Cytogenetics and palynology of this new species are never reported before. Therefore, this study aimed to study the taxonomical study, karyology and palynology of Kaempferia pseudoparviflora sp. nov. from Thailand. Materials and Methods: Plant taxonomy study was surveyed to collect specimen, described, measurements reported and illustrated from living specimens and spirit specimens under stereo microscope. Photographed were taken in the field. This specimen was compared with dry specimens at aboard herbaria. The chromosome numbers of this species were studied from root tips and counted from 20 cells. The nomenclature of the chromosome morphology, karyotype symmetry degree. For the arrangement of the karyotypes, the following parameters frommetaphase chromosomes were calculated by methods of several scientists. The pollens of this species were analyzed using an alcohol series. The pollen grains morphology were studied using SEM. Results: The new species has four distinctive in leaf, pseudostem, peduncle and inflorescence. The morphological characters of Kaempferia pseudoparviflora are similar to those of K. parviflora. A cytogenetic study of this species was found that chromosome number 2n = 22 and the karyotype formula to be symmetrical with 8+14 m and one visible satellite chromosome. The pollen morphology of a new species was monad, spheroidal, 99.37±3.61 µm in diam., large size, radial symmetry, apolar, inaperturate, exine sculpturing verrucate and regulate. Conclusion: Kaempferia pseudoparviflora was discovered to be a new species and endemic to Thailand.