RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR)

Abstract

Background: RNF5 is an ERAD E3 ligase targeting CFTR to co-translational degradation, and RNF185 is an RNF5 homolog. Results: RNF185 targets CFTR and CFTRαF508 to co-translational degradation and synergizes with RNF5 to their posttranslational degradation. Conclusion: RNF185 and RNF5 act together as major ERAD E3 ligases of CFTR. Significance: The RNF5/RNF185 module is a new potential therapeutic target for the treatment of cystic fibrosis. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.