Chiral separation of catechin by capillary electrophoresis using mono-, di-, tri-succinyl-β-cyclodextrin as chiral selectors

Abstract

The chiral separation of (±)-catechin was investigated by capillary electrophoresis using characterized succinyl-β-cyclodextrins (Suc-β-CDs) with one to three degree of substitution values. The effects of nature and concentration of Suc-β-CDs and running buffer pH on the migration time and resolution of (±)-catechin are discussed. All three kinds of Suc-β-CDs show a clear baseline separation of (±)-catechin in capillary electrophoresis. Mono-Suc-β-CD effectively separated (±)-catechin, and additional substituted CDs (di- and tri-Suc-β-CD) were capable of chiral separation at a broad pH range. The optimum running conditions were found to be 100 mM borate buffer (pH 9.8) containing 5 mM mono-Suc-β-CD with no methanol organic modifier. © 2009 Wiley-Liss, Inc.