Antidote strategies to reverse anticoagulation with TB-402, a long-acting partial inhibitor of factor VIII

Abstract

Background: TB-402 is a partially inhibiting antibody of factor VIII that is under development as a long-acting anticoagulant. Patients and Methods: The reversibility of FVIII inhibition by TB-402 was evaluated in vitro after spiking with recombinant human FVIII (rhFVIII), human plasma-derived FVIII (hpdFVIII), recombinant activated human FVII (rhFVIIa), FVIII inhibitor bypassing activity (FEIBA), and prothrombin complex concentrate (PCC). Twelve subjects were randomized to placebo or 35 or 70IUkg-1 rhFVIII 48h after a single dose of 620μgkg-1 TB-402. TB-402 concentrations, FVIII activity (FVIII:C), activated partial thromboplastin time (APTT) and thrombin generation were measured over a period of 8 weeks. Results: In spiked samples, TB-402 inhibited FVIII:C by 30%, prolonged APTT by 4.5s, and reduced the peak height in the thrombin generation assay to 56%±13% of the control value. In the presence of 10μgmL-1 TB-402, rhFVIII restored FVIII:C and APTT to the values obtained in the absence of TB-402. The inhibitory effect of TB-402 on thrombin generation was entirely reversed by rhFVIII, hpdFVIII, rhFVIIa, FEIBA, and PCC. In men, the mean half-life (t1/2) of TB-402 was 14.2 days. TB-402 lowered the endogenous thrombin potential by 23% for ∼35 days. Infusion of 35IUkg-1 rhFVIII had a marginal effect, whereas 70IUkg-1 rhFVIII restored FVIII:C, reduced APTT back to baseline for 9h, and restored thrombin generation for ∼3h. Conclusions: TB-402 resulted in a stable long-term anticoagulant effect. rhFVIII and other procoagulants counteracted the effect of TB-402 temporarily, and may be effective antidotes for future clinical practice. © 2012 International Society on Thrombosis and Haemostasis.