Characterization of lipase gene fragment from alcaligenes sp. JG3 bacterium

Abstract

Objective: Degenerate PCR technique has been successfully used to identify a lipase gene fragment of Alcaligenes sp. JG3. Primers were designed based on lipase genes of bacterium Azospirillum sp. B510 and Alcaligenes faecalis using PrimerBlast software. Methodology: Sequence analysis of the fragment with a size of 0.4 kb amplified using the forward primer (AlF4) 5’-GTCTACAGCAATCCCAAGAC-3’ and reversed primer (AlR4): 5’-GGAGGGGTAAATCCACAGTT-3’ represented a 394 bp nucleotide sequence which has been submitted to NCBI GenBank with Accession No. of KP872319. Results: The obtained DNA sequence was confirmed as part of lipase gene as it shared 98% amino acid and 88.07% nucleotide similarity with lipase gene of Alcaligenes faecalis, that covered approximately 27% the gene. Conclusion: The designed primers based on lipase gene of Alcaligenes faecalis were able to amplify 394 bp lipase gene fragment of bacterial strain Alcaligenes sp. JG3.