Distribution of Human Granulocyte Colony‐stimulating Factor Receptors on Hematopoietic and Nonhematopoietic Tumor Cell Lines

Abstract

Introduction of two tyrosine residues into human granulocyte colony‐stimulating factor (G‐CSF) allowed us to obtain radioiodinated material efficiently. By using this material, specific receptors for human G‐CSF were analyzed. It was found that human G‐CSF receptors were expressed on several myelocytic and monocytic leukemia cell lines. Furthermore, they were also expressed on two chorio‐carcinoma cell lines. However, the human G‐CSF receptors were not detected on other nonhemato‐poietic tumor cell lines examined, indicating that expression of G‐CSF receptors is strictly limited as compared with receptors for granulocyte/macrophage colony‐stimulating factor (GM‐CSF), which are widely distributed on nonhematopoietic tumors. Copyright © 1990, Wiley Blackwell. All rights reserved