Minerals consumption by Acetobacter xylinum on cultivation medium on coconut water

Abstract

The objective of this work is to verifying the consume of the minerals K, Na, Fe, Mg, P, S-SO4-2, B, N Total Kjedahl (NTK), NO3--N, and NH4+-N in the production of bacterial cellulose by Acetobacter xylinum, according to the medium and the manner of cultivation. The fermentative process was in ripe and green coconut water. K and Na were determined by flame emission photometry, Mg and Fe by atomic absorption spectrophotometry, P by molecular absorption spectrophotometry, S-SO4-2 by barium sulphate turbidimetry, B by Azomethin-H method, NTK by Kjeldahl method, N-NO3- and N-NH4+ by vapor distillation with magnesium oxide and Devarda's alloy, respectively. In Fermentation of ripe coconut water there were higher consumption of K (69%), Fe (84, 3%), P (97, 4%), S-SO2-2 (64, 9%), B (56, 1%), N-NO3- (94, 7%) and N-NH4+ (95, 2%), whereas coconut water of green fruit the most consumed ions were Na (94, 5%), Mg (67, 7%) and NTK (56, 6%). The cultivation under agitation showed higher mineral consumption. The higher bacterial cellulose production, 6 g.L-1, was verified in the coconut water fermentative in ripe fruit, added KH2PO4, FeSO4 and NaH2PO4 kept under agitation. © 2013, Sociedade Brasileira de Microbiologia.