Production of Protease Enzyme from Bacillus Clausii Sm3.

Abstract

The objective of present study was to screen and isolate protease producing bacteria from soil samples collected from farm soil, garden soil of BIMTS college campus and oil spilled area of Burhanpur. Soil samples were serially diluted and 0.1ml of sample was spread on skim milk agar, at 37o C for 48 hrs. Total six bacterial colonies from garden soil showed clear zone around the colony indicating protease activity. Among these, GS-P4 isolate produced highest protease activity and was identified as Bacillus Sp. by morphological and biochemical test. Moreover, various physiological characters were studied like pH, temperature, fermentation time. The protease showed maximum activity at pH 7.4 and the temperature for maximum protease activity was found to be 60oC. The unknown concentration of crude protease was determined using tyrosine standard curve and found to be 0.14µmole. Maximum activity of protease enzyme was 0.0233U/ml. The isolated protease also give better result of washing with detergent. Hence it can be added in detergent. The above results indicate that these bacterial isolate can be use as biotechnological tool for industrial purpose. Introduction