RecA-independent DNA damage induction of Mycobacterium tuberculosis ruvC despite an appropriately located SOS box

Abstract

Mycobacterium tuberculosis ruvC was induced by DNA damage in a △recA strain despite having an appropriately positioned SOS box to which LexA binds in vitro. An inducible transcript start mapped within the SOS box, and transcriptional fusions identified the promoter. Disruption of the SOS box did not prevent induction, indicating that an alternative mechanism plays a significant role in the control of ruvC expression. Copyright © 2010, American Society for Microbiology. All Rights Reserved.