Genotyping of Toxoplasma gondii strains detected in pork sausage

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Abstract

DNA was extracted from 70 pork sausage samples obtained from 55 commercial establishments in the city of Botucatu, Sao Paulo, Brazil. Nested-polymerase chain reaction with primers specific for Toxoplasma gondii SAG2 locus was used and detected the parasite in 19 (27.14%) sausage samples. Digestion of amplicons with restriction enzymes HhaI and Sau3AI showed that 14 (73.68%) samples contained parasites genotyped as Type I, and 5 (26.32%) as Type III.

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Silva, A. V. D. A., De Mendonça, A. O., Pezerico, S. B., Domingues, P. F., & Langoni, H. (2005). Genotyping of Toxoplasma gondii strains detected in pork sausage. Parasitologia Latinoamericana, 60(1–2), 65–68. https://doi.org/10.4067/s0717-77122005000100011

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