Characterisation of the nitrite reductase gene (NII1) and the nitrate-assimilation gene cluster of Stagonospora (Septoria) nodorum

Abstract

By sequencing downstream of the cloned nitrate reductase gene (NIA1) in the phytopathogenic fungus Stagonospora (Septoria) nodorum, a second open reading frame was found. Further analysis revealed this to be the nitrite reductase gene (NIII). Both genes are transcribed in the same direction, and are separated by an intergenic region of 829-bp. The coding sequence of NIII is interrupted by three small introns and corresponds to a predicted protein of 1141 amino acids in length. Consensus binding sites for regulatory proteins are present in the promoter region of NII1. There is no indication, however, from hybridisation or sequence analysis that the nitrate transporter gene is closely associated with the NIA1-NIII cluster, as has been found for a number of fungi.