Screening Of Potential Soil Bacteria For Detection Of Polyhydroxyalkanoate (PHA) Accumulation

Abstract

Polyhydroxyalkanoate (PHAs) have focused much attention because of their complete biodegradability and their material resemblance with synthetic plastics. For this purpose, the present study aims on isolation of those bacteria that have the potential to synthesize intracellular PHA and characterisation of PHA producing bacterial isolates so as to obtain the greatest PHA yield. 36 bacterial isolates were isolated and screened by Sudan black staining, Nile blue staining and Tributyrine hydrolysis. Out of 36 bacterial isolates, 8 isolates were screened as effective PHA producers. The selected isolates were identified phenotypically as Pseudomonas sp. EO1, Aneurinibacillus sp. EO8, Brevibacterium sp. EO9, Bacillus sp. EO11, Pseudomonas sp. EO18, Bacillus sp. LO1, Achromobacter sp. LO8, Pseudomonas sp. LO10.