Addendum: Recovery of cellulases from spent broth of sugar beet pulp fermentation by Trichoderma reesei

Abstract

The exo- and endo-glucanases of Trichoderma reesei were recovered after growth on sugar beet pulp by vacuum concentration followed by (NH4)2SO4 or acetone precipitation. Final respective recoveries were 53% and 57% for the exoglucanase and 41% and 42% for the endoglucanase. The resuspended acetone precipitates of both enzymes showed no loss of activity after 30 d at 4°C, pH 4.8 and in the presence of 0.5% sorbic acid. (NH4)2SO4-precipitated exoglucanase lost activity in the same period. © 1988 Oxford University Press.