Protease secretion by Giardia duodenalis trophozoites upon interaction with epithelial cells and its association with the parasite adhesion was studied in co-cultures of parasites with IEC6 epithelial cell monolayers in the presence or absence of protease inhibitors. Proteolytic activity in supernatants from trophozoites was enhanced when they were co-cultured with IEC6 cells. This activity was strongly inhibited by pre-incubation of live trophozoites with E-64 and TPCK and a concomitant inhibition of parasite adhesion to IEC6 cells was observed. These data suggest that trophozoites secrete cysteine-type proteases that play a role in the adhesion of G. duodenalis to epithelial cells.
CITATION STYLE
Rodríguez-Fuentes, G. B., Cedillo-Rivera, R., Fonseca-Liñán, R., Argüello-García, R., Muñoz, O., Ortega-Pierres, G., & Yépez-Mulia, L. (2006). Giardia duodenalis: Analysis of secreted proteases upon trophozoite-epithelial cell interaction in vitro. Memorias Do Instituto Oswaldo Cruz, 101(6), 693–696. https://doi.org/10.1590/S0074-02762006000600020
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