Isolation of 62 kDa protein with antioxidant properties from natural honey

Abstract

Fourteen natural honey samples from Libya, Sudan and Pakistan were evaluated for their antioxidant activity by employing 1,1-Diphenyl-2- picrylhydrazyl (DPPH) radical assay. The scavenging activity of honey samples were in the range of 18-32% when compared to control. A 62 kDa protein was isolated from honey by gel filtration chromatography followed by reverse phase HPLC showed significant radical scavenging activity. The research pointed out the antioxidative role of honey proteins and possibility of their contribution to the therapeutic value of the natural honey.