HO-1 participate in the protection of RES in rat heart suffered from hypothermic preservation

Abstract

Background. Resveratrol (RES) is a polyphenolic compound and natural phytoalexin that plays a potential role in various human diseases. Studies have confirmed that RES has an important function in cardioprotection. Objectives. To investigate the effect of RES on HO-1 protein expression in rat heart after different duration of hypothermic preservation. Material and methods. The Langendorff model of isolated rat heart was used. After being stored in 4°C different Celsior solution for 9 h, Sprague–Dawley rats hearts were divided into 6 groups randomly: control group, 9 h group, 3 µM RES group, 10 µM RES group, 30 µM RES group, and 100 µM RES group. The morphological changes of cardiomyocytes were detected with the hematoxylin & eosin (H&E) staining using a light microscope. The mRNA and protein expression of HO-1 were detected using reverse-transcription polymerase chain reaction (RT-PCR) and western blotting. Results. Compared with the control group, cardiomyocytes were obviously injured in the 9 h group and the protein and mRNA expression of HO-1 were obviously decreased. Compared with the 9 h group, the mRNA and protein expression of HO-1 were increased in dose-dependent manner in the 3 µM RES, 10 µM RES and 30 µM RES group. Compared with the 9 h group, rat myocardial injury was gradually alleviated in 3 µM RES, 10 µM RES and 30 µM RES groups. However, the rat myocardial injury in the 100 µM RES group showed no more obvious improvement than in the 30 µM RES group. Conclusions. In the isolated rat heart, RES protects cardiomyocytes against hypothermic preservation injury through increasing HO-1 protein expression.