PCR-Based Detection of Aflatoxogenic Strains of Aspergillus flavus Isolated from Poultry Feed

Abstract

Twenty five isolates of A. flavus were detected by UV light (365nm) and ammonia vapor procedures. Aflatoxigenic A. flavus on Coconut Agar Medium (CAM) colored the reverse of glass petri dish with blue -green under UV light and produce a pink to red color by exposure to ammonia vapor. The detection by fluorescent blue revealed that 13 (52%) of isolates were aflatoxigenic by produce fluorescent color under UV (365nm) light, and also 13 (52%) of isolates were aflatoxigenic (positive) by ammonia vapor test. The molecular assessment was done on 25 isolates of A. flavus by using aflR gene primers. Five isolates of aflatoxigenic A. flavus positive identified isolates by PCR were randomly selected to sequence and analyze by basic local alignment search tool analysis (BLAST) to confirm the aflatoxigenic strains. Five isolates were positive and confirmed approximately compatible (99%-100%) homology with other A. flavus strains on NCBI.