Quantitative resistance against an isolate of Leptosphaeria maculans (blackleg) in selected Canadian canola cultivars remains effective under increased temperatures

Abstract

Blackleg disease, caused by Leptosphaeria maculans, is a serious threat to canola production in western Canada. While specific major resistance (R) genes can be effective, they can also be eroded rapidly by a shift in pathogen race composition. Quantitative resistance (QR) has the potential to provide more durable, if less complete, protection. However, the effectiveness of QR may vary widely in the field. It has long been suspected that elevated temperatures may limit the expression of QR. To test this hypothesis, the infection development of blackleg was assessed on three common Canadian canola cultivars (74-44 BL, PV 530 G and 45H29) showing QR, with and without a heat treatment of 7 h daily exposure to 32 °C for 1 week during rosette to early flowering under controlled environment conditions. The impact of elevated temperature on the susceptibility to blackleg was compared with that of a moderate temperature with a 22 °C daytime high. A susceptible cultivar, Westar, was used as a control. When data from both temperatures were pooled, all three QR cultivars showed lower blackleg severity relative to Westar. Elevated temperatures increased blackleg severity in Westar only (in terms of the stem-lesion length from the inoculation of the first true-leaf petiole in trials involving Westar and 74-44 BL) and in pooled data for disease severity index in trials involving Westar, PV 530 G and 45H29. These findings suggest that the QR traits in 74-44 BL, PV 530 G and 45H29 are useful for blackleg management in western Canada, especially under warmer growing conditions when plants are at the rosette to early flowering stages.