Intensification of β-glucosidase enzyme production from Aspergillus Niger using extractive fermentation with an aqueous two-phase system

Abstract

An extractive fermentation approach in an aqueous two-phase system (ATPS) with wheat bran as a main substrate was used in this study to intensify the production of β-glucosidase enzyme (EC 3.2.1.21) from Aspergillus Niger NRRL 322. Possible parameters, such as phase concentration and incubation period, were evaluated based on enzyme production. The fungus expressed high enzyme production, i.e. 948 U/ml after 96 h at 180 RPM when PEG4000 (12% w/w) and potassium phosphate (14% w/w) were used. This strategy of extractive fermentation with ATPS shows an increase in β-glucosidase enzyme yield of 948 U/ml compared with submerged fermentation, which gives a yield of 790 U/ml. β-Glucosidase yield in salt phase also increased by 1.2-fold compared with the submerged fermentation under the same conditions.