Measuring Protein Expression in Tissue

Abstract

Reproducible protein expression measurement is important in many areas of tissue research and must address the inherently high biological variability across a single tissue section. Whole slide imaging is a new technology offering substantial improvements for both brightfield and fluorescence measurement of protein expression. In this article we discuss the latest developments in fluorescence and brightfield whole slide imaging as it applies to protein expression, and discuss how the 2 approaches differ and are complementary.