Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria

Abstract

Molecular techniques have the advantage that, they are rapid, less laborious, and more sensitive, specific and efficient compared to the conventional method of bacteria identification. This work aimed at Molecular Identification of Selected Multiple Antibiotic Resistance Bacteria Isolated from Poultry Droppings in Akure, Nigeria. Samples were plated on selective and differential media. Isolation and identification of bacteria were carried out using standard microbiological method, Antibiotics susceptibility test was determined by the disk diffusion method, Plasmid DNA extraction, profiling and curing, Genomic DNA extraction, Polymerase Chain Reaction (PCR), agarose gel electrophoresis, 16S rRNA gene sequence and phylogenetic analysis were carried out by standard method. Eight bacteria were selected; Proteus mirabilis, Salmonella typhi, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella sp. Escherichia coli, Shigella sp. and Klebsiella pneumonia, they were resistant to at least seven antibiotics. The result revealed that the plasmid size ranged from 8.60 to 128.00 kilobase pair (kbp) and the number of plasmid carried by bacteria ranged from 1 to 3 plasmids. Based on the 16s rRNA sequences, the following bacteria were identified Salmonella enterica subsp. enterica serovar Typhi str. CT18, Proteus mirabilis strain HI4320, Pseudomonas fluorescens SBW25, Staphylococcus cohnii subsp. cohnii strain 532 Contig16, Salmonella enterica subsp. enterica serovar Infantis, Escherichia coli str. K12 substr. DH10B, Shigella flexneri 2a str. 2457T and Klebsiella pneumoniae strain J1. The presence of multiple resistant bacteria and plasmid mediated resistance of human pathogenic bacteria isolated from poultry droppings in Akure is of great public health importance.