Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging

Abstract

Super-resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy-to-use sub-diffraction imaging method that achieves an almost 2-fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3-fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state-of-the-art commercial laser scanning microscope systems.