Antioxidant Potential of Kersen Leaves (Muntingia Calabura L.) Leaves to Increase Endogenous Glutathione Peroxidase (GPx) Enzymes in Diabetic Rats

Abstract

The prevalence of diabetes mellitus (DM) is increasing. DM can cause an imbalance between protective antioxidants and increased production of free radicals. One such antioxidant is the endogenous enzyme glutathione peroxidase (GPx). Kersen (Muntingia calabura L.) contains flavonoids which show antioxidant activity. The purpose of this study was to examine the potential antioxidant of Kersen Leaves (Muntingia Calabura L.) leaves to GPx Enzymes in Diabetic Rats. This research is included in an experimental study with a post-test design only with control category design. The subjects of this study were 36 male rats. The measure of GDP levels using the GOD-PAP enzymatic method, while GPx uses the UV method. Data were analyzed using paired-t-test and OneWay ANOVA test. Statistical test results with paired t test showed significant differences in GDP levels before and after treatment (p = 0.0001). In the OneWay ANOVA test there was a average different GPx levels in each category (p = 0.0001). The most effective steeping increases the GPx level is a dose of 750 mg / 200 gr BW.