Brazil nut 2S albumin was synthesized in a transgenic French bean seed with a promoter of the gene for canavalin, 7S globulin from Canavalia gladiata

Abstract

A gene for Brazil nut 2S albumin, a methionine-rich protein, was fused to a promoter region of the canavalin gene. This chimeric gene was introduced into shoot apices of embryonic axes of French bean by particle bombardment and seeds were obtained from plants regenerated from the bombarded axes. Protein in the transgenic seeds was analyzed by immunoblotting with an antiserum against Brazil nut 2S albumin. A 12-kDa immunoreactive polypeptide was detected and its amount in the seed was estimated to comprise less than 1% of the soluble protein.