Isolation, identification and characterization of a straw degrading Streptomyces griseorubens JSD-1

Abstract

To reutilize rice straw immediately during the agricultural production process, an actinomycete named JSD-1 was isolated from soil and rotten straw using multiple selective culture media. It was identified as Streptomyces griseorubens through polyphasic taxonomy consisting of morphological and physiological characterization combined with the result of 16S rDNA gene sequence and phylogenetic analysis. It also released a series of enzymes, including cellulase, pectinase, xylanase and ligninase simultaneously. According to the experiment of weight loss, JSD-1 decomposed about 76% of the straw in 30 days. Besides, it had the ability to resist certain plant diseases, such as Sclerotinia rot of melon and so on. It was obviously that JSD-1 could degrade rice straw effectively and had great potential of being applied to the utilization of straw resource.