Structure of the mouse Toll-like receptor 13 ectodomain in complex with a conserved sequence from bacterial 23S ribosomal RNA

Abstract

Toll-like receptor 13 (TLR13) recognizes a conserved 10-nucleotide sequence from bacterial 23S ribosomal RNA, and binding of TLR13 to the target rRNA molecule triggers immune responses. Recently, the crystal structure of the TLR13 ectodomain bound by a 13-nucleotide single-stranded RNA (ssRNA13) was determined by using the initial phases provided by a cryo-electron microscopy map of TLR13 in complex with a 25-nucleotide ssRNA (ssRNA25). This structural snapshot describes a unique method for solving the crystal structure of the TLR13-ssRNA13 complex based on medium-resolution reconstruction of TLR13-ssRNA25 cryo-electron microscopy images.