CYP82Y1 is n-methylcanadine 1-hydroxylase, a key noscapine biosynthetic enzyme in opium poppy

Abstract

Background: The biochemistry of noscapine metabolism in opium poppy has not been established. Results: CYP82Y1 catalyzes the efficient 1-hydroxylation of N-methylcanadine. Silencing CYP82Y1 reduced noscapine accumulation in opium poppy plants. Conclusion: CYP82Y1 is N-methylcanadine 1-hydroxylase and functions in noscapine biosynthesis. Significance: The enzyme catalyzing the first step in the conversion of N-methylcanadine to noscapine has been identified and characterized. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.