Recombinant human irisin regulated collagen II, matrix metalloproteinase‑13 and the Wnt/β‑catenin and NF‑κB signaling pathways in interleukin‑1β‑induced human SW1353 cells

Abstract

Osteoarthritis (OA) is a degenerative joint disease that seriously affects the quality of life of patients. Irisin has been reported to regulate bone metabolism via the cellular autocrine mechanism and play a protective role in rat OA. In the present study, a SW1353 chondrosarcoma cell line was treated with interleukin (IL)-1β and irisin. The present study evaluated cell viability, expression levels of collagen II (Col II) and matrix metalloproteinase-13 (MMP-13), and activity of the Wnt/β-catenin and NF-κB signaling pathways in treated SW1353 cells. The present results suggested that IL-1β could decrease Col II expression and increase MMP-13 expression at both the mRNA and protein levels, and also activate the Wnt/β-catenin and NF-κB signaling pathways in SW1353 cells. By contrast, irisin was identified to reverse the effects of IL-1β in IL-1β-induced SW1353 cells. The present results suggested that irisin treatment may have a cartilage-protective role in an IL-1β-induced SW1353 cell model.