A Review on Study and Comparison between 2D Gel Electrophoresis and Mass Spectrometry

Abstract

Protein structure prediction is an important area of life sciences. The Protein prophecy is unbreakable because of its complex structure. The main problem in proteomics is to estimate the several proteins available in cell structure or tissue sample. The protein-protein interaction is essential to study the behaviour of protein in a specific environment, and study molecular relationship in living systems. Therefore, large scale proteomics technologies are required to measure physical connection of proteins in living organisms. A combination of high resolution two-dimensional (2-D)gel electrophoresis, highly sensitive biological mass spectrometry , and the speedily growing protein and DNA databases has resulted for high throughput proteomics. This review concentrates on two main techniques of protein identification 2-D gel electrophoresis and High Throughput Mass Spectrometry.