In Vitro and In Vivo Biotinylation of Endothelial Cell Surface Proteins in the Pursuit of Targets for Vascular Therapies for Brain AVMs

Abstract

Identification of membrane proteins that are expressed on the arteriovenous malformation (AVM) endothelium post radiosurgery is of fundamental importance in developing a new treatment of brain AVMs. We successfully optimized and then employed in vitro and in vivo biotinylation methodology of murine cerebral endothelial cell cultures (bEnd.3) and the rat model of AVM to label membrane proteins. Those membrane proteins were then captured on streptavidin resin and identified using proteomics analysis. This is the first time that proteomics has been employed in the study of AVMs.