Phytochemistry of the unpolar extract of Carica papaya leaves and its antidengue activity

Abstract

Antidenge activity of the n-hexane, ethyl acetate and n-butanol extracts from the leaves of Carica papaya against four dengue virus serotypes (DENV1-4) were evaluated. As a result, the n-hexane extract showed the best antiviral activity on all denge virus with PNRT50 from 15.625 to 31.25 μg/ml. From n-hexane extract, nine compounds including cycloartenol (1), cycloart-23,25-dien-3β-ol (2), cycloart-25-ene-3β,24β-diol (3), cycloart-25-ene-3β,24α-diol (4), 25-hydroxycycloart-23-ene-3-one (5), β-sitosterol (6), stigmasterol (7), nonacosan-1-ol (8), all-trans-polyprenol-12 (9) were isolated. Structural elucidation of the above compounds was supported by analysis of their spectral data. Compounds 2-5, 8 and 9 were isolated for the first time from the plant Carica papaya.