Serological response of patients infected with Salmonella typhi

Abstract

Aims - To evaluate a rapid immunoblotting procedure for providing evidence of infection with Salmonella typhi using 73 sera from patients infected with S typhi. Methods - A sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/immunoblotting procedure using lipopolysaccharide (LPS, O=9,12) and flagellar (H=d) antigens was used. Results - Seventy two of 73 sera contained antibodies to LPS, 40 sera also contained antibodies to H=d flagellar antigens. Analysis of acute and convalescent sera showed that only 62% of patients produced antibodies to flagellar antigens. Conclusions - The SDS-PAGE/immunoblotting procedure provided a rapid method for providing serological evidence of infection with S typhi.