Abstract
A reliable method for the introduction of foreign DNA into epidermal cells is described. Electroporation of murine BALB c MK-1 epidermal cells with pSV2-CAT resulted in the transient expression of chloramphenicol-acetyltransferase (0.03 to 0.05 nmoles acetylchloramphenicol per mg protein per min) in the transfected cells. Transfection of MK-1 cells with pSV2-neo led to the appearance of approximately eight G418 resistant clones per 10-6 cells per μg of plasmid DNA. Distinct patterns of integration of SV2-neo were detected in three different resistant clones. © 1986.
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CITATION STYLE
Reiss, M., Jastreboff, M. M., Bertino, J. R., & Narayanan, R. (1986). DNA-mediated gene transfer into epidermal cells using electroporation. Biochemical and Biophysical Research Communications, 137(1), 244–249. https://doi.org/10.1016/0006-291X(86)91202-7
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