Avaliação da toxicidade aguda, efeitos citotóxico e espasmolítico de Pomacea lineata (Spix, 1827) (Mollusca, Caenogastropoda)

Abstract

Pomacea sp. and its eggs are used against diarrhea and respiratory diseases in folk medicine. The aim of this study was to investigate acute toxicity, cytotoxic and spasmolytic activity of lyopbilized Pomacea lineata and its eggs. P. lineata and its eggs present no acute toxicity (until 2 g/kg p.o.) or cytotoxic effect (until 1 mg/mL). P. lineata and its eggs have no effect on guinea-pig trachea spontaneous tonus. P. lineata relaxed trachea pre-contracted with CCh in the presence (Emax = 48.8 ¦ 6,4 %) and absence (Emax = 47.3 ¦ 9,1 %) of epithelium, the eggs relaxed only in the presence (Emax = 36.3 ¦ 2.5 %) P. lineata was more potent to inhibit contractions induced by ACh (logIC50 = 2.5 ¦ 0.04 ?g/mL) than histamine (logIC50 = 2.7 ¦ 0.04 ?g/mL). The eggs inhibited contractions induced by ACh (logIC50 = 2.5 ¦ 0.02 ?g/mL) and histamine (logIC50 = 2.5 ¦ 0.06 ?g/mL) in a non-selective manner. P. lineata was more potent in relax ileum pre-contracted with ACh (logEC50 = 1.7 ¦ 0.12 ?g/mL) than KCl (logEC50 = 2.4 ¦ 0.06 ?g/mL) or histamine (logEC 50 = 2.2 ¦ 0.18 ?g/mL). The eggs were equipotent in relax ileum pre-contracted with of KCl (logEC50 = 2.3 ¦ 0.15 ?g/mL), ACh (logEC50 = 1.9 ¦ 0.14 ?g/ mL) or histamine (logEC50 = 2.2 ¦ 0.16 ?g/mL), that is suggestive of the blockade of the voltage-operated calcium channels. Collectively, the results validate folk use of P. lineata and its eggs to treat diarrhea and respiratory diseases