Photocontrol of Gibberellin Metabolism in Situ in Maize

Abstract

Mature maize seeds were labeled with 10 to 100 pg per seed of [(3)H] gibberellins (GA) and [(3)H]GA glucosyl conjugate-like substances by feeding [(3)H]GA(20) of high specific activity (2.3 Curies per millimole) during seed maturation. The dry seeds, which contained 14% [(3)H]GA(20), 7% putative [(3)H]GA(1) and 78% [(3)H]GA glucosyl conjugate-like metabolites, were imbibed and germinated in the dark and under incandescent light. In both light and dark the proportion of [(3)H]GA conjugate-like metabolities declined (relative to that in the mature dry seeds) during imbibition and up to germination at hour 36. This decline was accompanied by increases in the proportions of [(3)H]GA(20) and putative [(3)H]GA(1) thereby indicating hydrolysis, which was greater in the dark than in the light. The proportions of [(3)H]GA conjugate-like substances in light-grown germinants were higher (121 and 141% of dark-grown) at 24 and 48 hour harvests and this statistically significant pattern was sustained up to 120 hours after imbibition. Conversely, the proportions of [(3)H]GA(20) and putative [(3)H]GA(1) were lower in the light-grown seedlings. Thus, during imbibition, hydrolysis (de-conjugation) of [(3)H]GA glucosyl conjugate-like substances apparently occurred, and occurred more rapidly in the dark than in the light. Subsequently, during germination the reformation of [(3)H]GA conjugate-like substances was less rapid in the dark than in the light. The observation that dark-imbibed seeds and dark-grown seedlings have higher proportions of putative free [(3)H]GAs, relative to [(3)H]GA conjugate-like substances, is consistent with the increased shoot elongation (etiolation) that occurs in dark-grown maize seedlings, and may indicate a homeostatic role for GAs and their conjugates in shoot elongation of maize germinants.