Skin mucus of gilthead sea bream (sparus aurata l.). protein mapping and regulation in chronically stressed fish

58Citations
Citations of this article
120Readers
Mendeley users who have this article in their library.

Abstract

The skin mucus of gilthead sea bream was mapped by one-dimensional gel electrophoresis followed by liquid chromatography coupled to high resolution mass spectrometry using a quadrupole time-of-flight mass analyzer. More than 2,000 proteins were identified with a protein score filter of 30. The identified proteins were represented in 418 canonical pathways of the Ingenuity Pathway software. After filtering by canonical pathway overlapping, the retained proteins were clustered in three groups. The mitochondrial cluster contained 59 proteins related to oxidative phosphorylation and mitochondrial dysfunction. The second cluster contained 79 proteins related to antigen presentation and protein ubiquitination pathways. The third cluster contained 257 proteins where proteins related to protein synthesis, cellular assembly, and epithelial integrity were over-represented. The latter group also included acute phase response, signaling. In parallel, two-dimensional gel electrophoresis methodology identified six proteins spots of different protein abundance when comparing unstressed fish with chronically stressed fish in an experimental model that mimicked daily farming activities. The major changes were associated with a higher abundance of cytokeratin 8 in the skin mucus proteome of stressed fish, which was confirmed by immunoblotting. Thus, the increased abundance of markers of skin epithelial turnover results in a promising indicator of chronic stress in fish.

Cite

CITATION STYLE

APA

Pérez-Sánchez, J., Terova, G., Simó-Mirabet, P., Rimoldi, S., Folkedal, O., Calduch-Giner, J. A., … Sitjà-Bobadilla, A. (2017). Skin mucus of gilthead sea bream (sparus aurata l.). protein mapping and regulation in chronically stressed fish. Frontiers in Physiology, 8. https://doi.org/10.3389/fphys.2017.00034

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free