Treatment of established graft versus host disease in dogs by antithymocyte serum or prednisone

Abstract

Marrow grafts after 1200 R of whole body irradiation were carried out between pairs of unrelated dogs of different breeds that were incompatible for dog histocompatibility antigens. Three groups of recipients were studied and recipients in all groups were treated with the immunosuppressive agent methotrexate (MTX), 0.4 mg/kg, on days 1, 3, 6, and 11, and then once weekly until day 102 after grafting. Dogs in group 1 were given MTX only. 30 of 35 recipients died between 11 and 51 days and 5 survived more than 102 days. Median survival time was 20 days. Dogs in group 2 were treated with rabbit antidog antithymocyte serum (ATS) when graft versus host (GVH) disease became established. 8 of 10 recipients died between 19 and 76 days and 2 survived more than 102 days. Median survival time was 45 days. Dogs in group 3 were treated with daily injections of prednisone when GVH disease became apparent. 7 of 9 recipients died between 11 and 34 days and 2 survived more than 102 days. Median survival time was 24 days. It was concluded that established GVH disease in dogs with histoincompatible marrow grafts can be favorably influenced by ATS with significant (p < 0.01) prolongation of survival. However, there was no evidence that treatment with ATS increased the fraction of long term survivors in this histoincompatible setting. Prednisone was ineffective and did not prolong survival (p > 0.1).