Abstract
There are a few PHA-producer bacteria that can uptake glycerol to produce this biopolymer. Among them, Bacillus megaterium LVN01 has demonstrated to be able to grow up using glycerol as a carbon source. Glycerol dehydrogenase (GD) plays a key role in the synthesis of PHA from glycerol. In this study, the improvement of glycerol uptake by a recombinant strain of B. megaterium carrying pHT01-bmgd was evaluated in order to enhance PHA production. The biomass and PHA production were evaluated and compared to wild-type. It was determined that the PHA produced by both strains was PHB and the highest improvement in PHB yield was 226% at 30 h.
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Cardozo, J. R. G., Bucheli, R. V., Sánchez, C. del C., Riesco, I. de la M., & Martínez, A. L. M. (2019). Engineering of Bacillus megaterium for improving PHA production from glycerol. Asia-Pacific Journal of Molecular Biology and Biotechnology, 27(3), 64–72. https://doi.org/10.35118/apjmbb.2019.027.3.07
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