Ultrasound-assisted extraction of fucoxanthin from Sargassum angustifolium and Cystoseira indica brown algae

Abstract

The ultrasound-assisted extraction (UAE; 150 W, 100 amplitude) of fucoxanthin from Sargassum angustifolium and Cystoseira indica brown algae was investigated and compared with conventional extraction methods. Different solvents (methanol, acetone, and methanol/acetone) and sonication times (1, 5, 10, and 15 min) were applied. Among a variety of solvents, methanol extract of C. indica (0.77 ± 0.05 mg/g) and methanol/acetone extract of S. angustifolium (0.70 ± 0.02 mg/g) was more effective in fucoxanthin extraction. UAE had a significant effect on yield extraction and 15 min sonication had a high recovery yield about 0.79 ± 0.01 mg/g and 0.81 ± 0.01 (mg/g) from S. angustifolium and C. indica methanolic extracts, respectively. Moreover, the crude extract of C. indica exhibited inhibitory growth against Escherichia coli and Staphylococcus aureus, while S. angustifolium extract had weak antimicrobial activity. According to the gas chromatography results, γ-Linolenic acid and arachidonic acid were detected as main PUFA in S. angustifolium and C. indica, respectively. Novelty impact statement: Ultrasound-assisted extraction increased the recovery yield of fucoxanthin from the Persian Gulf brown seaweeds Sargassum angustifolium and Cystoseira indica. The best solvents for fucoxanthin extraction from C. indica and S. angustifolium were methanol and methanol/acetone, respectively. The antibacterial activity of C. indica extract was better than S. angustifolium extract.