In vitro propagation of endangered Iris species

Abstract

Calli from Iris petrana and I. atrofusca were produced from flower bases, leaf bases and anthers. The flower bases responded positively to the culture conditions and gave the best results. Callus induction medium was Murashige and Skoog (MS) supplemented with 4.5 μM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5 μM Kinetin and 4.5 μM 1-naphthaleneacetic acid (NAA). Formation of green structures was noticed on calli of both species after transferring calli to callus culture medium (MS supplemented with 4.5 μM N6-benzyladenine (BA), 0.45 μM 2,4-D and 4.5 μM NAA). Embryo-like structures were appeared after transferring calli to embryogenesis induction medium (MS supplemented with 4.5 μM 2,4-D, 0.5 μM Kinetin, 4.5 μM NAA and 300 mg L-1 proline). Both green and albino plantlets were observed after calli were transferred to regeneration medium {MS supplemented with 4.5 μM BA, 0.45 μM 2,4-D and 0.49 μM indol-3-butyric acid (IBA)}. © 2006 Asian Network for Scientific Information.