MIRG survey 2011: Snapshot of rapidly evolving label-free technologies used for characterizing molecular interactions

Abstract

The field of label-free biophysical technologies used to quantitatively characterize macromolecular interactions with each other and with small molecules has grown enormously in the last 10 years. The most widely used analytical technologies for characterizing biomolecular interactions are surface plasmon resonance (SPR), isothermal titration calorimetry (ITC), biolayer interferometry (BLI),andanalytical ultracentrifugation(AUC).Measuring interaction parameters accuratelyandquantitatively is challenging, as it requires specialized expertise, training, and instrumentation. The Molecular Interaction Research Group (MIRG)conductedanonline surveydesignedto capture the current profile of label-free technologies, including ITC,SPR,and otherbiosensorsusedinacademiaandthepharmaceuticalindustrysector.Themaingoalofthesurveywastotakeasnapshot of laboratory, instrumentation, applications for measuring various biophysical parameters, confidence in data interpretation, data validation and acceptability, and limitations of using various technologies. Through this survey, we anticipate that the participating laboratories will be able to gauge theirowncapabilities and gain insights into the relative success of the different technologies that they use for characterizing molecular interactions. © 2012 ABRF.