Anti-Ageing Potential of a Cream (W/O Emulsion) Containing Grape Seed Extract (GSE): Formulation and in vivo Evaluation of Effectiveness Using Non-Invasive Biophysical Technique

Abstract

In this study, topical water in oil W/O emulsion containing 3% grape seed extract (GSE) was developed and evaluated its anti-aging effects in comparison with its vehicle (base) as control. Both base and selected formulation were stored at 8°C ± 0.1°C, 25°C ± 0.1°C, 40°C ± 0.1°C, 40°C ± 0.1°C with 75% relative humidity (RH) for time duration of 12 weeks in order to evaluate their physiochemical stability. With an objective to determine its anti-aging potential & safety of the formulation containing GSE, various skin parameters such as moisture level, pH value, sebum content, mean pore size, elasticity and roughness were evaluated by using non-invasive biophysical techniques. Both base and formulation were applied on human cheeks of 20 healthy female between ages of 28-58 for a period of 12 weeks. Panelists also answered a preformed questionnaire to assess performance of the product. The results of the non-invasive biophysical test have shown that the product increases skin moisture, elasticity, and smoothness significantly (p<0.05). In addition, product also decreases skin roughness and mean pore sizes significantly (p<0.05) while the base shows insignificant result (p>0.05).