El caseinato de sodio (CasNa) incrementa el número de linfocitos B en ratón

Abstract

Introduction: Sodium caseinate (CasNa), a salt of casein is a proinflammatory agent in mice, and it is able to induce granulopoiesis in vivo and increase the production of cytokines which is key for this biological process. Objective: To assess whether CasNa is able to induce a biological effect on cells from lymphoid origin and the production of cytokines involved in this lineage in vivo. Materials and methods: Female BALB /c mice from 8 to 12 weeks old were used. The animals were inoculated intraperitoneally (IP) with 1 ml of CasNa (10% PBS w / v) 4 times every 48 h. The B cell populations and the incorporation of BrdU were analyzed by flow cytometry. Detection of Interleukin-7 was assessed by ELISA technique. Results: We established that after IP inoculation, the number of B lymphocytes 220+ from the spleen of mice treated with CasNa increased compared to those that only received the vehicle (89.01 ± 1.03 vs 75.66 ± 2.08) respectively as a treatment, and the incorporation of BrdU in B220 + cells (38.59 ± 4.48 vs 11.82 ± 1.04 respectively). We also established that the concentration of Interleukin-7 (IL-7) in the serum of treated mice with CasNa increased compared to those that only received the vehicle (62.1 ± 17.5 vs 26.9 ± 4.4 pg / ml). Conclusion: CasNa is able to increase the number of B lymphocytes in the spleen and also induces IL-7 production, a cytokine that is key for the B cell lymphopoiesis.