A full factorial design (ascorbic acid/L-cysteine inhibitors, temperature, and time as factors) study was conducted to enhance inhibition of polyphenol oxidase (PPO) activity without decreasing cocoa polyphenol concentrations. The data obtained were modelled through a new equation, represented by T, which correlates both high polyphenol content with reduced specific PPO activity. At optimized values (70 mM inhibitory solution at 96 °C for 6.4 min, Γ=11.6), 93.3% PPO inhibition and total polyphenol of 94.9 mg GAE/g were obtained. In addition, microscopy images confirmed the cell morphological changes measured as the fractal dimension and explained the possible cell lysis and denaturation as a result of heat treatment and chemical inhibitors. Results also showed that PPO enzyme was most suitable (higher vmax/Km ratio) for catechol, witha reduction in its affinity of 13.7-fold after the inhibition heat treatment. Overall, this work proposed a suitable and food-safe procedure for obtaining enriched polyphenol extract with low enzyme activity.
CITATION STYLE
Toro-Uribe, S., Godoy-Chivatá, J., Renévillamizar-Jaimes, A., Perea-Flores, M. de J., & López-Giraldo, L. J. (2020). Insight of polyphenol oxidase enzyme inhibition and total polyphenol recovery from cocoa beans. Antioxidants, 9(6). https://doi.org/10.3390/antiox9060458
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