Antibacterial activity of kecombrang fruit simplicia ekstract (nicolaia speciosa) against gram positive bacteria staphylococcus aureus fncc 0047 in vitro

Abstract

The survival of microorganisms depends on the environment. Unhealthy environment causes food intoxication problems resulting in food poisioning. One alternative that can be done to prevent the occurrence of food poisioning by using traditional medicines made from natural ingredients, especially kecombrang fruit simplicia extract. This study aims to determine the activity ofkecombrang fruit simplicia extract against Staphylococcus aureus FNCC 0047 bacteria. The design used was a Completely Randomized Design (CRD) which was arranged with 10 treatment factors and repeated 3 times, resulting in 30 experimental units. The factors studied were the concentration of the kecombrang fruit simplicia extract consisting of concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%. The variable observed in this study was the antibacterial activity ofS.aureus FNCC 0047 with the well-diffusion method. The data obtained were analyzed by parametric analysis of one way analysis of variance (ANOVA) at the level of 5% then significant results were continued with Duncan Multiple Range Test (DMRT) at the level of 5%. Kecombrang fruit simplicia extract has antibacterial activity against the growth of Gram positive S. aureus FNCC 0047 bacteria with the most effective concentration in inhibiting the growth ofS.aureus FNCC 0047 bacteria is 40%. In this case, the extract of the kecombrang fruit simplicia has the ability to inhibit the growth ofS. aureus FNCC 0047 bacteria.