Nitrobacter winogradskyi transcriptomic response to low and high ammonium concentrations

Abstract

Nitrobacter winogradskyi Nb-255 is a nitrite-oxidizing bacterium that can grow solely on nitrite (NO2-) as a source of energy and nitrogen. In most natural situations, NO2- oxidation is coupled closely to ammonium (NH4+) oxidation by bacteria and archaea and, conceptually, N. winogradskyi can save energy using NH4+ to meet its N-biosynthetic requirements. Interestingly, NH4+ delayed the growth of N. winogradskyi when at concentrations higher than 35 mM, but grew well at concentrations below 25mMNH4+ while adjusting the expression of 24% of its genes. Notable genes that changed in expression included those with roles in nitrogen and carbon assimilation. Contrary to expectations, higher expression of glutamate synthase (GOGAT), instead of glutamate dehydrogenase, was detected at higher NH4+ concentration. Genes in assimilatory NO2- metabolism and the degradation of glycogen and biofilm/motility were downregulated when N. winogradskyi was grown in the presence of NH4+. Nitrobacter winogradskyi grown in medium with 25mMNH4+ upregulated genes in post-translational modification, protein turnover, biogenesis and chaperons. The data suggest that N. winogradskyi physiology is modified in the presence of NH4+ and is likely to be modified during coupled nitrification with NH3 oxidizers.