Efficient expression of bovine α‐lactalbumin in Saccharomyces cerevisiae

Abstract

A synthetic gene encoding the mature bovine α‐lactalbumin fused to the preproregion of the yeast α‐mating factor has been expressed and secreted at high level in Saccharomyces cerevisiae under the control of the α‐mating promotor. Growth conditions were found to be critical for the expression: recombinant α‐lactalbumin could only be detected in the medium provided the culture was grown at neutral pH. The secreted bovine α‐lactalbumin is enzymatically active and identical to the whey protein, as confirmed by SDS/PAGE, IEF, ultraviolet and CD spectral analysis, and amino‐terminal sequence determination. Copyright © 1991, Wiley Blackwell. All rights reserved