Discovery of bioactive compounds in the Chinese herbal formula NaoShuanTong Capsule (NSTC) against hemorheological disorders

Abstract

NaoShuanTong capsule (NSTC) has been commonly used to treat blood circulation disorders and neurological dysfunction against cerebral ischemia in China. In this study, using an acute blood stasis rat model, canonical correlation analysis between chemical high performance liquid chromatography (HPLC) fingerprint and in vivo biotests was conducted to discover and identify the bioactive compounds responsible for NSTC’s protective effects on hemorheological disorders. Four independent effect components were extracted from the 12 originally detected parameters. Three bioactive compounds were found to have significant contribution in two or more effect components against hemorheological disorders. Typhaneoside could influence the erythrocyte aggregation and coagulation function. Isorhamnetin-3-O-neohesperidoside had impacts on the erythrocyte aggregation, erythrocyte deformability and platelet aggregation. Benzoyl-oxypaeoniflorin was associated with erythrocyte aggregation, coagulation function and platelet aggregation. The findings could offer useful references for NSTC’s quality control and clinical practice. AbbreviationsAdr adrenalineASP aspirinAPTT activated partial thromboplastin timeCCA canonical correlation analysisEAI erythrocyte aggregation indexEDI erythrocyte deformability indexEEI erythrocyte electrophoresis indexHCT haematokritHPLC high performance liquid chromatographyMPAR maximum platelet aggregation rateNSTC NaoShuanTong CapsulePCA principal component analysisPT Pollen TyphaePT prothrombin timePV plasma viscosityRC Radix CurcumaeRG Rhizome GastrodiaeRPR Radix Paeoniae RubraRR Radix RhaponticiTCM traditional Chinese medicineWBV whole blood viscosit.