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Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay

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Abstract

Genetic variants of Thailand orthohantavirus (THAIV) have been recently reported from rodents in South-East Asia and in islands from the South-West part of the Indian Ocean. In order to detect THAIV and its variants, we developed a sensitive and specific real-time RT-PCR targeting the S segment. Our assay was developed in two different RT-PCR systems that gave similar results in terms of sensitivity. Moreover, our results demonstrated a specificity of 100%.

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Raharinosy, V., Heraud, J. M., Rabemananjara, H. A., Telfer, S., Rakoto, D. A. D., Filippone, C., & Reynes, J. M. (2019). Fast, sensitive and specific detection of Thailand orthohantavirus and its variants using one-step real-time reverse-transcription polymerase chain reaction assay. Viruses, 11(8). https://doi.org/10.3390/v11080718

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